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% Viral and Total Protein ratios of H1N1pdm09 and <t> H7N9 </t> vaccines <xref ref-type= a ." width="250" height="auto" />
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% Viral and Total Protein ratios of H1N1pdm09 and H7N9 vaccines <xref ref-type= a ." width="100%" height="100%">

Journal: PLoS ONE

Article Title: Immunogenicity and Protective Efficacy of a Vero Cell Culture-Derived Whole-Virus H7N9 Vaccine in Mice and Guinea Pigs

doi: 10.1371/journal.pone.0113963

Figure Lengend Snippet: % Viral and Total Protein ratios of H1N1pdm09 and H7N9 vaccines a .

Article Snippet: NAi antibodies were detected using an enzyme-linked lectin assay, essentially as previously described [ ], using a recombinant A/Anhui/1/2013 (H7N9) N9 NA (Sino Biological Inc.; Cat. no. 40108-VNAHC; Lot no. LC07AP2401) or a recombinant A/California/04/2009 (H1N1pdm09) N1 NA (Sino Biological Inc.; Cat. no. 1158-VNAHC; Lot no. LC04NO1601).

Techniques:

HA and NA antibody responses induced by H7N9 and H1N1pdm09 vaccines in guinea pigs.

Journal: PLoS ONE

Article Title: Immunogenicity and Protective Efficacy of a Vero Cell Culture-Derived Whole-Virus H7N9 Vaccine in Mice and Guinea Pigs

doi: 10.1371/journal.pone.0113963

Figure Lengend Snippet: HA and NA antibody responses induced by H7N9 and H1N1pdm09 vaccines in guinea pigs.

Article Snippet: NAi antibodies were detected using an enzyme-linked lectin assay, essentially as previously described [ ], using a recombinant A/Anhui/1/2013 (H7N9) N9 NA (Sino Biological Inc.; Cat. no. 40108-VNAHC; Lot no. LC07AP2401) or a recombinant A/California/04/2009 (H1N1pdm09) N1 NA (Sino Biological Inc.; Cat. no. 1158-VNAHC; Lot no. LC04NO1601).

Techniques:

HA and NA antibody responses induced by H7N9 and H1N1pdm09 vaccines in DBA/2J mice.

Journal: PLoS ONE

Article Title: Immunogenicity and Protective Efficacy of a Vero Cell Culture-Derived Whole-Virus H7N9 Vaccine in Mice and Guinea Pigs

doi: 10.1371/journal.pone.0113963

Figure Lengend Snippet: HA and NA antibody responses induced by H7N9 and H1N1pdm09 vaccines in DBA/2J mice.

Article Snippet: NAi antibodies were detected using an enzyme-linked lectin assay, essentially as previously described [ ], using a recombinant A/Anhui/1/2013 (H7N9) N9 NA (Sino Biological Inc.; Cat. no. 40108-VNAHC; Lot no. LC07AP2401) or a recombinant A/California/04/2009 (H1N1pdm09) N1 NA (Sino Biological Inc.; Cat. no. 1158-VNAHC; Lot no. LC04NO1601).

Techniques:

Dose-dependent survival of DBA/2J mice immunized with whole-virus H7N9 vaccine against lethal challenge with wild-type H7N9 virus.

Journal: PLoS ONE

Article Title: Immunogenicity and Protective Efficacy of a Vero Cell Culture-Derived Whole-Virus H7N9 Vaccine in Mice and Guinea Pigs

doi: 10.1371/journal.pone.0113963

Figure Lengend Snippet: Dose-dependent survival of DBA/2J mice immunized with whole-virus H7N9 vaccine against lethal challenge with wild-type H7N9 virus.

Article Snippet: NAi antibodies were detected using an enzyme-linked lectin assay, essentially as previously described [ ], using a recombinant A/Anhui/1/2013 (H7N9) N9 NA (Sino Biological Inc.; Cat. no. 40108-VNAHC; Lot no. LC07AP2401) or a recombinant A/California/04/2009 (H1N1pdm09) N1 NA (Sino Biological Inc.; Cat. no. 1158-VNAHC; Lot no. LC04NO1601).

Techniques:

DBA/2J mice were immunized with whole-virus (WV) H7N9 vaccine or buffer as control on days 0 and 21. Spleen cells were collected 7 days after the first, or 21 days after the booster immunization (i.e. 42 days after the first), and stimulated with whole-virus (WV) H7N9 vaccine or recombinant H7 HA (rHA) for the determination of cells responding to the antigens by secretion of either IFN-γ (A) or IL-4 (B) using an ELISPOT assay. Statistically significant differences of comparisons between T cell responses in mice receiving H7N9 vaccine or buffer are shown. H7 HA-specific IgG subclass responses were analyzed by ELISA using sera collected on day 42 (C). IgG subclass responses on day 42 were compared by ANOVA. ***, p<0.0001; ns, not significant.

Journal: PLoS ONE

Article Title: Immunogenicity and Protective Efficacy of a Vero Cell Culture-Derived Whole-Virus H7N9 Vaccine in Mice and Guinea Pigs

doi: 10.1371/journal.pone.0113963

Figure Lengend Snippet: DBA/2J mice were immunized with whole-virus (WV) H7N9 vaccine or buffer as control on days 0 and 21. Spleen cells were collected 7 days after the first, or 21 days after the booster immunization (i.e. 42 days after the first), and stimulated with whole-virus (WV) H7N9 vaccine or recombinant H7 HA (rHA) for the determination of cells responding to the antigens by secretion of either IFN-γ (A) or IL-4 (B) using an ELISPOT assay. Statistically significant differences of comparisons between T cell responses in mice receiving H7N9 vaccine or buffer are shown. H7 HA-specific IgG subclass responses were analyzed by ELISA using sera collected on day 42 (C). IgG subclass responses on day 42 were compared by ANOVA. ***, p<0.0001; ns, not significant.

Article Snippet: NAi antibodies were detected using an enzyme-linked lectin assay, essentially as previously described [ ], using a recombinant A/Anhui/1/2013 (H7N9) N9 NA (Sino Biological Inc.; Cat. no. 40108-VNAHC; Lot no. LC07AP2401) or a recombinant A/California/04/2009 (H1N1pdm09) N1 NA (Sino Biological Inc.; Cat. no. 1158-VNAHC; Lot no. LC04NO1601).

Techniques: Recombinant, Enzyme-linked Immunospot, Enzyme-linked Immunosorbent Assay